EEA1

Gene Symbol EEA1
Entrez Gene 8411
Alt Symbol MST105, MSTP105, ZFYVE2
Species Human
Gene Type protein-coding
Description early endosome antigen 1
Other Description early endosome antigen 1, 162kD|early endosome-associated protein|endosome-associated protein p162|zinc finger FYVE domain-containing protein 2
Swissprots Q14221 Q15075
Accessions EAW97471 EAW97472 Q15075 AF173389 AAQ13630 AK292008 BAF84697 AK307918 BC017837 AAH17837 BC041930 BC156545 AAI56546 BC172504 AAI72504 BF515615 BP228986 BX648463 L40157 AAA79121 X78998 CAA55632 XM_011538814 XP_011537116 XM_011538815 XP_011537117 XM_011538816 XP_011537118 NM_003566 NP_003557
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Subcellular Location Cytoplasm. Early endosome membrane; Peripheral membrane protein.
Top Pathways Endocytosis, Phagosome, Tuberculosis

EEA1 (C45B10) Rabbit mAb - 3288 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C45B10
Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.

EEA1 Antibody - 2411 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using EEA1 Antibody.

EEA1 (E-8) - sc-365652 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EEA1 (E-8): sc-365652. Western blot analysis of EEA1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.

EEA1 (G-4) - sc-137130 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EEA1 (G-4): sc-137130. Western blot analysis of EEA1 expression in NIH/3T3 (A) and HeLa (B) whole cell lysates.

EEA1 (H-300) - sc-33585 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EEA1 (H-300): sc-33585. Western blot analysis of EEA1 expression in HeLa whole cell lysate.

EEA1 (N-19) - sc-6415 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of EEA1 expression in A-431 (A,C) and NIH/3T3 (B,D) whole cell lysates. Antibodies tested include EEA1 (C-15): sc-6414 (A,B) and EEA1 (N-19): sc-6415 (C,D).

EEA1 (281.7) - sc-53939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EEA1 (281.7): sc-53939. Western blot analysis of EEA1 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.

EEA1 (C-15) - sc-6414 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EEA1 (C-15): sc-6414. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.

Anti-EEA1 antibody - ab50313 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."

Anti-EEA1 antibody - ab90557 from Abcam

Prices $376.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Anti-EEA1 antibody (ab90557) at 1/500 dilution + Recombinant EEA1 (immunogen) at 0.2 µg

Anti-EEA1 antibody - ab123380 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEA1 antibody (ab123380) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : HL60 cell extract

Anti-EEA1 antibody - ab28503 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-EEA1 antibody (ab28503) at 0.06 µg/ml + Placenta tissue lysate.SecondaryHRP conjugated anti-Rabbit IgG diluted 1/50000 - 100000

Anti-EEA1 antibody - ab86566 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEA1 antibody (ab86566) at 0.04 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

Anti-EEA1 antibody [1G11] - Early Endosome Marker - ab70521 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G11
ICC/IF image of ab70521 stained HepG2 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70521 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml.

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker - ab109110 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4245
All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilutionLane 1 : COS-1 cell lysateLane 2 : NIH 3T3 cell lysateLane 3 : C6 cell lysateLane 4 : HeLa cell lysateLane 5 : Jurkat cell lysateLane 6 : JAR cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (Alexa Fluor® 488) - ab185039 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4245
Overlay histogram showing HeLa cells stained with ab185039 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185039, 1/50 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (Alexa Fluor® 647) - ab196186 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4245
Overlay histogram showing Jurkat cells stained with ab196186 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196186, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (HRP) - ab195553 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4245
All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (HRP) (ab195553) at 1/5000 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-EEA1 antibody [mAbcam18175] - ab18175 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam18175
All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryRabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilutionPerformed under reducing conditions.

Anti-EEA1 antibody [mAbcam18175] (FITC) - ab150269 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam18175
Overlay histogram showing HeLa cells stained with ab150269 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150269, 1μg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (FITC) (ab91368, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-EEA1 antibody - C-terminal - ab137403 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLane 4 : HeLa S3 whole cell lysateLane 5 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-EEA1 antibody - Early Endosome Marker - ab2900 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of rabbit polyclonal to EEA1 (ab2900, 1/200; Alexa Fluor 488 1/200; green) and monoclonal mouse anti-b-tubulin-II (1/400; Alexa Fluor 568 1/200; red). Magnification 63x ; insert 180x.

Anti-EEA1 Antibody - LS-C65197 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-EEA1 Antibody (N-Terminus) - LS-C203204 from LifeSpan Bioscience

Prices $245.00, $325.00
Sizes 40 µg, 100 µg
Host Rabbit
Clonality Polyclonal

Anti-EEA1 Antibody (aa35-84) - LS-C116773 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EEA1 antibody LS-C116773 Western blot of HepG2 cell lysate.
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