Anti-EEA1 antibody [mAbcam18175]
| Name | Anti-EEA1 antibody [mAbcam18175] |
|---|---|
| Supplier | Abcam |
| Catalog | ab18175 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | mAbcam18175 |
| Applications | WB FC |
| Species Reactivities | Mouse, Human, Rat, Chicken, Bovine, Dog, Chimpanzee, Zebrafish |
| Antigen | Synthetic peptide derived from within residues 1350 to the C-terminus of Human EEA1 |
| Blocking Peptide | Human EEA1 peptide |
| Description | Mouse Monoclonal |
| Gene | EEA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryRabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11739_ab18175%2520_1.jpg)
All lanes : Anti-EEA1 antibody [mAbcam18175] (ab18175) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 20 µg per lane.SecondaryRabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilutionPerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab18175 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11740_EEA1-Primary-antibodies-ab18175-8.jpg)
Overlay histogram showing HeLa cells stained with ab18175 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
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Liu J, Han C, Xie B, Wu Y, Liu S, Chen K, Xia M, Zhang Y, Song L, Li Z, Zhang T, Ma F, Wang Q, Wang J, Deng K, Zhuang Y, Wu X, Yu Y, Xu T, Cao X. Nat Immunol. 2014 Jul;15(7):612-22.
Analysis of the subcellular phosphoproteome using a novel phosphoproteomic - Analysis of the subcellular phosphoproteome using a novel phosphoproteomic
Zhou H, Elisma F, Denis NJ, Wright TG, Tian R, Zhou H, Hou W, Zou H, Figeys D. J Proteome Res. 2010 Mar 5;9(3):1279-88.