![Overlay histogram showing HeLa cells stained with ab150269 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150269, 1μg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (FITC) (ab91368, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11741_ab150269-220235-ab150269AP1849085FC.jpg)
Overlay histogram showing HeLa cells stained with ab150269 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150269, 1μg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (FITC) (ab91368, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.