All lanes : Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLane 4 : HeLa S3 whole cell lysateLane 5 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-EEA1 antibody - C-terminal (ab137403) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Confocal immunofluorescence analysis of methanol-fixed HeLa cells labelling EEA1 with ab137403 at 1/500 (Green). Alpha-tubulin filaments are labeled in Red.