Anti-EEA1 antibody - Early Endosome Marker

• Supplier: Abcam
• Catalog: ab2900
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC IHC-P WB ICC/IF IP IHC-F
• Species Reactivities: Mouse, Rat, Chicken, Hamster, Bovine, Dog, Human, Xenopus, Zebrafish, Monkey
• Antigen: Synthetic peptide derived from within residues 1350 to the C-terminus of Human EEA1
• Description: Rabbit Polyclonal
• Gene: EEA1
• Conjugate: Unconjugated

Product images

Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of rabbit polyclonal to EEA1 (ab2900, 1/200; Alexa Fluor 488 1/200; green) and monoclonal mouse anti-b-tubulin-II (1/400; Alexa Fluor 568 1/200; red). Magnification 63x ; insert 180x.

ICC/IF image of ab2900 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2900, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

ab2900 Rabbit polyclonal to EEA1 (1/500) was used in fixed HEK cells that had been transfected with Rab5-GFP. Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling rabbit polyclonal to EEA1 ab2900 (1/500; Alexa Fluor 568; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 5µm. Showing little co-localisation as expected. Immunofluorescence microscopy revealed a typically punctuate staining of EEA1 in the HEK cells.

Immunolocalisation of EEA1 in HeLa cells using ab2900.

All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/1000 dilutionLane 1 : HeLa nuclearLane 2 : HeLa whole cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor anti rabbit at 1/50000 dilutionPerformed under reducing conditions.

All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilutionLane 1 : HEK293 Whole Cell lysateLane 2 : HEK293 Whole Cell lysate with Human EEA1 peptide (ab14946) at 1 µgLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionPerformed under reducing conditions.

All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/mlLane 1 : Xenopus laevis lysateLane 2 : Mouse 3T3 cell lysateLane 3 : Mouse brain cell lysateLane 4 : Mouse liver cell lysateLane 5 : Rat brain cell lysateLane 6 : Rat liver cell lysateLane 7 : Dog lysateLane 8 : CHO cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionPerformed under reducing conditions.

Image courtesy of Human Protein Atlas    Paraffin embedded sections of human prostate tissue were incubated with ab2900 (1/500 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab2900 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

ab2900 staining mouse L-cells by ICC/IF.  Cells were formaldehyde fixed, permeabilized in Triton X-100 and incubated with ab2900 diluted 1/1000 for 1 hour at 37°C.  A Cy2® conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

Product References

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