Anti-EHD1 antibody [EPR4955]

• Supplier: Abcam
• Catalog: ab109747
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4955
• Applications: WB IP FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to internal sequence amino acids 390-415 of Human EHD1
• Description: Rabbit Monoclonal
• Gene: EHD1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : JAR cell lysateLysates/proteins at 10 µg per lane.

ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.

Product References

GRAF1 promotes ferlin-dependent myoblast fusion. - GRAF1 promotes ferlin-dependent myoblast fusion.

Lenhart KC, Becherer AL, Li J, Xiao X, McNally EM, Mack CP, Taylor JM. Dev Biol. 2014 Sep 15;393(2):298-311.