Anti-EIF2S1 antibody

• Supplier: Abcam
• Catalog: ab26197
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human, Drosophila, Rat
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eIF2 alpha
• Description: Rabbit Polyclonal
• Gene: eIF-2alpha
• Conjugate: Unconjugated

Product images

All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ICC/IF image of ab26197 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab26197, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Image courtesy of Human Protein Atlasab26197 staining eiF2 alpha in human small intestine. The paraffin embedded tissue was incubated with ab26197 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab26197 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Product References

Therapeutic modulation of eIF2alpha phosphorylation rescues TDP-43 toxicity in - Therapeutic modulation of eIF2alpha phosphorylation rescues TDP-43 toxicity in

Kim HJ, Raphael AR, LaDow ES, McGurk L, Weber RA, Trojanowski JQ, Lee VM, Finkbeiner S, Gitler AD, Bonini NM. Nat Genet. 2014 Feb;46(2):152-60.

Regulation of sumo mRNA during endoplasmic reticulum stress. - Regulation of sumo mRNA during endoplasmic reticulum stress.

Moore KA, Plant JJ, Gaddam D, Craft J, Hollien J. PLoS One. 2013 Sep 18;8(9):e75723.

IRES-mediated translation of cellular messenger RNA operates in eIF2alpha- - IRES-mediated translation of cellular messenger RNA operates in eIF2alpha-

Thakor N, Holcik M. Nucleic Acids Res. 2012 Jan;40(2):541-52.

Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2alpha - Pkh1 interacts with and phosphorylates components of the yeast Gcn2/eIF2alpha

Kimpe M, Voordeckers K, Thevelein JM, Van Zeebroeck G. Biochem Biophys Res Commun. 2012 Mar 2;419(1):89-94. doi:

Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in - Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in

Ye J, Cardona M, Llovera M, Comella JX, Sanchis D. J Mol Cell Cardiol. 2012 Oct;53(4):578-87.

Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and - Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and

Farny NG, Kedersha NL, Silver PA. RNA. 2009 Oct;15(10):1814-21.