eIF-2alpha

Gene Symbol eIF-2alpha
Entrez Gene 32617
Alt Symbol CG9946, Dmel\CG9946, EIF-2alpha, Eif2alpha, IF2A_DROME, M(1)14C, M(1)19-153, M(1)8e3-10, deIF2alpha, eIF-2, eIF-2 a, eIF-2 alpha, eIF-2alphaeIF-2alpha, eIF2 alpha, eIF2-alpha, eIF2a, eIF2alpha, eiF-2alpha, elF2alpha, l(1)14Cf, l(1)19-153
Species Drosophila
Gene Type protein-coding
Description eukaryotic translation Initiation Factor 2alpha
Other Description CG9946-PA|CG9946-PB|eIF-2alpha-PA|eIF-2alpha-PB|eukaryotic initiation factor 2alpha|eukaryotic translation initiation factor 2|eukaryotic translation initiation factor 2alpha|minute (1) 14C
Swissprots P41374 Q9V3G2
Accessions AAF48615 AHN59799 P41374 AF145633 AAD38608 L19196 AAA53627 NM_001298400 NP_001285329 NM_132902 NP_573130
Function eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This preinitiation complex mediates ribosomal recognition of a start codon during the scanning process of the leader region.
Top Pathways Protein processing in endoplasmic reticulum, RNA transport

Anti-EIF2S1 (phospho S51) antibody [E90] - ab32157 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone E90
Overlay histogram showing HeLa cells stained with ab32157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32157, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-EIF2S1 antibody - ab26197 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
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