Western blot analysis of p21 expression in untreated (A,C,E) and phorbol ester-induced (B,D,F) C32 nuclear extracts. Antibodies tested include p21 (187): sc-817 (A,B), p21 (C-19)-G: sc-397-G (C,D) and p21 (C-19): sc-397 (E,F).
p21 (C-19)-G: sc-397-G. Western blot analysis of p21 expression in HeLa nuclear extract.
p21 (C-19): sc-397. Western blot analysis of p21 expression in non-transfected 293T: sc-117752 (A), mouse p21 transfected 293T: sc-122305 (B) and C32 (C) whole cell lysates.
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant Akt1 treated (B,E) and human recombinant Akt1 and lambda protein phosphatase treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (Thr 145)-R: sc-20220-R (A,B,C) and p21 (C-19): sc-397 (D,E,F).
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (C-8): sc-377514 (A,B,C) and p21 (C-19): sc-397 (D,E,F).
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (D-4): sc-377515 (A,B,C) and p21 (C-19): sc-397 (D,E,F).
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (Ser 146)-R: sc-12902-R (A,B,C) and p21 (C-19): sc-397 (D,E,F).
Western blot analysis of p21 expression in C32 nuclear extracts (A,B). Antibodies tested include p21 (H-164): sc-756 (A) and p21 (C-19)-G: sc-397-G (B).