CDKN1A

Gene Symbol CDKN1A
Entrez Gene 1026
Alt Symbol CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Other Description CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1
Swissprots Q9BUT4 Q14010 Q6FI05 P38936
Accessions AAM11787 CAJ98559 CAJ98560 CBX54345 EAX03904 EAX03905 EAX03906 EAX03907 P38936 AA534613 AB209881 BAD93118 AB451290 BAG70104 AB451422 BAG70236 AI973138 AK298901 BAG61011 AK309512 AM392711 CAL37589 AM392920 CAL37798 BC000275 AAH00275 BC000312 AAH00312 BC001935 AAH01935 BC013967 AAH13967 BQ941282 BT006719 AAP35365 BU154329 BU193165 CR536533 CAG38770 DA552678 DA582789 DA699672 DA702185 DA838987 L25610 AAA16109 L26165 AAA19811 L47232 AAB59559 L47233 AAB59560 S67388 AAB29246 U03106 AAC04313 U09579 AAA85641 NM_000389 NP_000380 NM_001220777 NP_001207706 NM_001220778 NP_001207707 NM_001291549 NP_001278478 NM_078467 NP_510867
Function May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. {ECO:0000269|PubMed:8242751, ECO:0000269|PubMed:9106657}.
Subcellular Location Cytoplasm. Nucleus.
Tissue Specificity Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
Top Pathways HTLV-I infection, MicroRNAs in cancer, Epstein-Barr virus infection, Oxytocin signaling pathway, Proteoglycans in cancer

p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 8587 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Confocal immunofluorescent analysis of MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) #3465 (green). Actin filaments were labeled with DY-544 phalloidin (red).

p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 11850 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Confocal immunofluorescent analysis of MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) #3465 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

p21 Waf1/Cip1 (12D1) Rabbit mAb (PE Conjugate) - 8865 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Flow cytometric analysis of Jurkat (blue) and MCF7 (green) cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (PE Conjugate).

p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 8493 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Confocal immunofluorescent analysis of MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) #3465 (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

p21 Waf1/Cip1 (12D1) Rabbit mAb (Biotinylated) - 8831 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Western blot analysis of extracts from COS-7, HeLa, and MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 5487 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Confocal immunofluorescent analysis of MCF7 cells using p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

p21 Waf1/Cip1 (12D1) Rabbit mAb - 2947 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 12D1
Western blot analysis of extracts from various cell types using p21 Waf1/Cip1 (12D1) Rabbit mAb.

p21 Waf1/Cip1 (DCS60) Mouse mAb - 2946 from Cell Signaling Technology

Prices $99.00, $246.00, $596.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots), 300 µl (60 western blots)
Host Mouse
Clonality Monoclonal
Clone DCS60
Western blot analysis of extracts from 293, MCF-7 and COS cells, using p21 Waf1/Cip1 (DCS60) Mouse mAb.

p21 (B-2) - sc-271532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (B-2): sc-271532. Western blot analysis of p21 expression in non-transfected: sc-117752 (A) and mouse p21 transfected: sc-122305 (B) 293T whole cell lysates.

p21 (C-19) - sc-397 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of p21 expression in untreated (A,C,E) and phorbol ester-induced (B,D,F) C32 nuclear extracts. Antibodies tested include p21 (187): sc-817 (A,B), p21 (C-19)-G: sc-397-G (C,D) and p21 (C-19): sc-397 (E,F).

p21 (F-8) - sc-271610 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (F-8): sc-271610. Western blot analysis of p21 expression in non-transfected 293T: sc-117752 (A), mouse p21 transfected 293T: sc-122305 (B) and C32 (C) whole cell lysates.

p21 (DCS-60) - sc-56335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (DCS-60): sc-56335. Western blot analysis of p21 expression in Raji whole cell lysate.

p21 (H-164) - sc-756 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of p21 expression in C32 nuclear extracts (A,B). Antibodies tested include p21 (H-164): sc-756 (A) and p21 (C-19)-G: sc-397-G (B).

p21 (F-5) - sc-6246 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (F-5): sc-6246. Western blot analysis of p21 expression in KNRK (A) and C32 (B) nuclear extracts.

p21 (M-19) - sc-471 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of human (h) and mouse (m) p21 expressed in E. coli. Antibodies tested at 1:5,000 include p21 (M-19): sc-471, p21 (N-20): sc-469 and p21 (C-19): sc-397.

p21 (AC8.3) - sc-53393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (AC8.3): sc-53393. Western blot analysis of p21 expression in MCF7 (A), C32 (B) and phorbol-treated C32 (C) nuclear extracts.

p21 (187) - sc-817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (187): sc-817. Western blot analysis of human (h) and mouse (m) p21 expressed in E. coli. Tested at 1:20,000 against both human (h) and mouse (m) p21 at 100, 20, 10 and 5 ng per lane.

p21 (N-20) - sc-469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p21 (N-20)-G: sc-469-G. Western blot analysis of p21 expression in C32 (A) and phorbol-treated C32 (B) nuclear extracts.

p21 (SX118) - sc-53870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (SX118): sc-53870. Western blot analysis of p21 expression in C-32 whole cell lysate.

p21 (WA-1) - sc-51689 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (WA-1): sc-51689. Western blot analysis of p21 expression in untreated C32 (A) and PMA treated C32 (B) nuclear extracts.

p21 (SPM306) - sc-65595 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (SPM306): sc-65595. Western blot analysis of p21 expression in C32 (A) and Raji (B) whole cell lysates.

p21 (0.N.488) - sc-71811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p21 (0.N.488): sc-71811. Western blot analysis of p21 expression in C32 whole cell lysate.

p-p21 (C-8) - sc-377514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase (sc-200312A) treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (C-8): sc-377514 (A,B,C) and p21 (C-19): sc-397 (D,E,F).

p-p21 (D-6) - sc-377569 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of p21 phosphorylation in untreated (A,D), Akt1 treated (B,E) and Akt1 and lambda protein phosphatase (sc-200312A) treated (C,F) p21 fusion proteins. Antibodies tested include p-p21 (D-6): sc-377569. (A,B,C) and p21 (C-19): sc-397 (D,E,F).

p-p21 (D-4) - sc-377515 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of p21 phosphorylation in untreated (A,D), human recombinant PKC α treated (B,E) and human recombinant PKC α and lambda protein phosphatase (sc-200312) treated (C,F) human recombinant p21 fusion proteins. Antibodies tested include p-p21 (D-4): sc-377515 (A,B,C) and p21 (C-19): sc-397 (D,E,F).
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