Anti-EIF3S1 antibody [EPR16161]

• Supplier: Abcam
• Catalog: ab196018
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16161
• Applications: IHC-P WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EIF3S1 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: EIF3J
• Conjugate: Unconjugated

Product images

All lanes : Anti-EIF3S1 antibody [EPR16161] (ab196018) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human chronic tonsillitis tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human chronic tonsillitis tissue is observed. Counter stained with Hematoxylin.Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Rat cerebral cortex is observed. Counter stained with Hematoxylin.Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling EIF3S1 with ab196018 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

EIF3S1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196018 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196018 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196018 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196018 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.