EIF3J

Gene Symbol EIF3J
Entrez Gene 8669
Alt Symbol EIF3S1, eIF3-alpha, eIF3-p35
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 3, subunit J
Other Description eukaryotic translation initiation factor 3 subunit J|eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
Swissprots Q9UI65 O75822 B4DUI3 Q9H8Q2 Q9BUD2 F5H425
Accessions EAW77268 EAW77269 EAW77270 EAW77271 O75822 AF090923 AAF24039 AK002006 AK023388 BAB14555 AK300659 BAG62345 AL536561 BC002719 AAH02719 BU621047 CN414611 U97670 AAC78729 NM_001284335 NP_001271264 NM_001284336 NP_001271265 NM_003758 NP_003749
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
Subcellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03009}.
Top Pathways RNA transport

eIF3J (D21G7) XP ® Rabbit mAb - 8161 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21G7
Western blot analysis of extracts from various cell lines using eIF3J (D21G7) XP ® Rabbit mAb.

eIF3J Antibody - 3261 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using eIF3J Antibody.

eIF3α (C-20) - sc-16355 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF3α (C-20): sc-16355. Western blot analysis of eIF3α expression in Jurkat whole cell lysate.

eIF3α (H-1) - sc-376651 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF3α (H-1): sc-376651. Western blot analysis of eIF3α expression in MES-SA/Dx5 (A) and Jurkat (B) whole cell lysates.

eIF3α (H-250) - sc-50356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
eIF3α (H-250): sc-50356. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Anti-EIF3S1 antibody - ab68243 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-EIF3S1 antibody (ab68243) at 1/500 dilutionLane 1 : EIF3S1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-EIF3S1 antibody [EPR16161] - ab196018 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16161
All lanes : Anti-EIF3S1 antibody [EPR16161] (ab196018) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-EIF3S1 antibody - Carboxyterminal end - ab71416 from Abcam

Prices $359.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-EIF3S1 antibody - Carboxyterminal end (ab71416) at 1/60 dilution + K562 cell line lysates at 35 µg

Anti-EIF3J Antibody (HRP) - LS-C183163 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EIF3J Antibody (FITC) - LS-C183164 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EIF3J Antibody (Biotin) - LS-C183165 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EIF3J Antibody - LS-C189976 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-EIF3J Antibody - LS-C307369 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-EIF3J Antibody (C-Terminus) - LS-C151942 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF3J antibody Western Blot of MCF7 cell lysate. EIF3J is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.

Anti-EIF3J Antibody (aa53-102) - LS-C151943 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF3J antibody Western Blot of Jurkat cell lysate. EIF3J is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.

Anti-EIF3J Antibody (aa208-258) - LS-C288528 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse eIF3J/EIF3S1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 used at a dilution of 1:1000 (1 Detection: DAB.

Anti-EIF3J Antibody (aa230-280) - LS-C148532 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of eIF3J in 293 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) 3T3 cell lysate using LS-C148532 at 1.0 ug/ml, 1.0 ug/ml and 0.5 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Anti-EIF3J Antibody (aa208-258) - LS-C286825 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse eIF3J/EIF3S1 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-eIF3J/EIF3S1 used at a dilution of 1:100.

EIF3J Antibody - NBP2-24572 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3J Antibody [NBP2-24572] - Analysis of eIF3J in 293 lysate in the 1) absence and 2) presence of immunizing peptide and 3) 3T3 lysate using eIF3J antibody at 1.0 ug/ml, 1.0 ug/ml and 0.5 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

EIF3J Antibody - NB100-93287 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3J Antibody [NB100-93287] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-93287 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

EIF3J Antibody - NBP2-13953 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3J Antibody [NBP2-13953] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

EIF3J Antibody - NBP2-16301 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3J Antibody [NBP2-16301] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.

EIF3J Antibody - H00008669-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

EIF3J Antibody - H00008669-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: EIF3J Antibody [H00008669-B01] - analysis of EIF3S1 expression in transfected 293T cell line by EIF3S1 MaxPab polyclonal antibody (B01). Lane 1: EIF3S1 transfected lysate (29.10 KDa). Lane 2: Non-transfected lysate.

EIF3J Antibody - H00008669-A01 from Novus Biologicals

Host Mouse
Clonality Polyclonal
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