ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.
ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).
All lanes : Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilutionLane 1 : A549 cell lysateLane 2 : A431 cell lysateLane 3 : HepG2 cell lysateLane 4 : HEK293 cell lysateLane 5 : HeLa cell lysateLane 6 : Jurkat cell lysateLane 7 : K562 cell lysateLane 8 : NIH3T3 cell lysateLane 9 : MCF-7 cell lysate
Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution + eIF4B recombinant fragment (aa 381-585)