EIF4B

Gene Symbol EIF4B
Entrez Gene 1975
Alt Symbol EIF-4B, PRO1843
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 4B
Other Description -
Swissprots Q6GPH5 P23588 Q8WYK5 Q4G0E3 Q6IB46 Q53HQ2 B4DS13
Accessions CAA39265 EAW96657 P23588 AB076839 BAB82380 AF119854 AAF69608 AK092188 AK222528 BAD96248 AK293731 BAG57157 AK299329 BAG61335 AK299510 BAG61463 AK299526 BAG61475 AK307496 AK310244 AK312187 BAG35120 BC001097 AAH01097 BC007745 BC013994 BC047302 BC061907 BC064029 BC073139 AAH73139 BC073154 AAH73154 BC098437 AAH98437 BG547613 BP236621 BP243230 BU431305 BU902688 CR456958 CAG33239 CR627377 CAH10474 DC403237 XM_006719274 XP_006719337 NM_018507 NM_001300821 NP_001287750 NM_001417 NP_001408
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Top Pathways RNA transport, PI3K-Akt signaling pathway, Proteoglycans in cancer, mTOR signaling pathway

Phospho-eIF4B (Ser406) Antibody - 5399 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or λ phosphatase treated, using Phospho-eIF4B (Ser406) Antibody (upper) or eIF4B Antibody #3592 (lower).

Phospho-eIF4B (Ser422) Antibody - 3591 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following siRNA transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated.

Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb - 8151 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1C10
Western blot analysis of extracts from HeLa cells, untreated (-) or λ phosphatase-treated (+), using Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb (upper) and eIF4B Antibody #3592 (lower).

eIF4B (1F5) Mouse mAb - 13088 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1F5
Western blot analysis of extracts from PANC-1, HeLa, and RD cells using eIF4B (1F5) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

eIF4B Antibody - 3592 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, 293 and NIH/3T3 cells using eIF4B Antibody.

eIF4B (D-4) - sc-376062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF4B (D-4): sc-376062. Western blot analysis of eIF4B expression in Ramos whole cell lysate.

eIF4B (D-16) - sc-82587 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF4B (D-16): sc-82587. Western blot analysis of eIF4B expression in non-transfected 293T: sc-117752 (A), human eIF4B transfected 293T: sc-111557 (B) and Ramos (C) whole cell lysates.

eIF4B (A-2) - sc-390912 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF4B (A-2): sc-390912. Western blot analysis of eIF4B expression in Ramos (A) and MCF7 (B) whole cell lysates.

eIF4B (C-15) - sc-82586 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF4B (C-15): sc-82586. Western blot analysis of eIF4B expression in HeLa whole cell lysate.

p-eIF4B (Ser 422) - sc-293101 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-eIF4B (Ser 422): sc-293101. Western blot analysis of eIF4B phosphorylation expression in untreated (A) and EGF treated (B) HeLa whole cell lysates.

Anti-eIF4B (phospho S406) antibody [EPR7683] - ab134138 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7683
All lanes : Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) at 1/10000 dilutionLane 1 : HeLa cell lysate untreatedLane 2 : HeLa cell lysate treated with Alkaline PhosphataseLysates/proteins at 10 µg per lane.SecondaryGoat Ani-rabbit HRP at 1/2000 dilution

Anti-eIF4B (phospho S422) antibody - ab59300 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilutionLane 1 : extracts from NIH/3T3cellsLane 2 : extracts from NIH/3T3cells with immunizing peptide

Anti-eIF4B (phospho S504) antibody [EP2261Y] - ab75823 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2261Y
All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilutionLane 1 : 293T cell lysate, untreatedLane 2 : 293T cell lysate, treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-eIF4B antibody - ab186856 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling eIF4B with ab186856 at 1/200. Magnification: 400x.

Anti-eIF4B antibody - ab38359 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eIF4B antibody (ab38359) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 Human embryonic kidney cell line Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-eIF4B antibody - ab71057 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eIF4B antibody (ab71057) at 1/50 dilutionLane 1 : 293 cell lysate, non transfected Lane 2 : 293 cell lysate, transiently transfected with the eIF4B gene Lysates/proteins at 2 µg per lane.Observed band size : 79 kDa (why is the actual band size different from the predicted?)

Anti-eIF4B antibody - ab66780 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-eIF4B antibody (ab66780) at 1.25 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-eIF4B antibody - ab50103 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-eIF4B antibody (ab50103) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-eIF4B antibody - ab171820 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-eIF4B antibody (ab171820) at 1 µg/mlLane 1 : eIF4B-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-eIF4B antibody [1F5] - ab129369 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F5
ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.

Anti-eIF4B antibody [EP2299Y] - ab68474 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2299Y
Anti-eIF4B antibody [EP2299Y] (ab68474) at 1/200000 dilution + HeLa cell lysate at 10 µgSecondaryHRP conjugated Goat anti-rabbit. at 1/2000 dilution

Anti-eIF4B antibody - C-terminal - ab171012 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-eIF4B antibody - C-terminal (ab171012) at 1 µg/ml + Mouse brain lysate at 10 µg

Anti-EIF4B Antibody (N-Terminus) - LS-C6731 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF4B Antibody (aa1-200) - LS-C36281 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-EIF4B Antibody (aa490-539) - LS-C31324 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
EIF4B antibody LS-C31324 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.
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