Anti-eIF4E (phospho S209) antibody [EP2151Y]

• Supplier: Abcam
• Catalog: ab76256
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP2151Y
• Applications: WB IP IHC-P ICC/IF
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: A synthetic peptide corresponding to residues surrounding Serine 209 of human eIF4E
• Description: Rabbit Monoclonal
• Gene: EIF4E
• Conjugate: Unconjugated

Product images

ab76256 at 1/100 dilution staining eIF4E in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

All lanes : Anti-eIF4E (phospho S209) antibody [EP2151Y] (ab76256) at 1/10000 dilutionLane 1 : 293 cell lysates, untreatedLane 2 : 293 cell lysates, treated with APLysates/proteins at 10 µg per lane.

ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 (ab120365), by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of ab120365 (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Product References

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