Anti-eIF4E antibody

• Supplier: Abcam
• Catalog: ab1126
• Prices: $400.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP WB IHC-P ELISA ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant full length protein (Human)
• Description: Rabbit Polyclonal
• Gene: EIF4E
• Conjugate: Unconjugated

Product images

Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract

ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.See Abreview

IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not - The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not

Mittal S, Aslam A, Doidge R, Medica R, Winkler GS. Mol Biol Cell. 2011 Mar 15;22(6):748-58.

Activation of cap-independent translation by variant eukaryotic initiation factor - Activation of cap-independent translation by variant eukaryotic initiation factor

Kaiser C, Dobrikova EY, Bradrick SS, Shveygert M, Herbert JT, Gromeier M. RNA. 2008 Oct;14(10):2170-82.