Anti-eIF4E antibody [5D11]

• Supplier: Abcam
• Catalog: ab171091
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 5D11
• Applications: ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Human
• Antigen: Recombinant full length protein corresponding to Human eIF4E
• Description: Mouse Monoclonal
• Gene: EIF4E
• Conjugate: Unconjugated

Product images

ab171091 staining eIF4E (green) in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

ab171091 staining eIF4E (green) in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

All lanes : Anti-eIF4E antibody [5D11] (ab171091) at 1/1000 dilutionLane 1 : MCF7 whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : K562 whole cell lysateLane 4 : Jurkat whole cell lysateLane 5 : U2OS whole cell lysateLane 6 : HepG2 whole cell lysateLane 7 : C2C12 whole cell lysateLane 8 : NIH3T3 whole cell lysateLane 9 : NRK whole cell lysateLysates/proteins at 80 µg per lane.SecondaryGoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique

Immunoprecipitation of eIF4E from HeLa whole cell lysate using ab171091 at 3µg per 500µg lysate. Lane 1Primary: ab117091, 1/1000 dilutionSample: Hela whole cell lysate