Anti-eIF4E antibody [Y449]

• Supplier: Abcam
• Catalog: ab33768
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y449
• Applications: WB IHC-P FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in the C terminus of Human elf 4E
• Description: Rabbit Monoclonal
• Gene: EIF4E
• Conjugate: Unconjugated

Product images

Anti-eIF4E antibody [Y449] (ab33768) at 1/20000 dilution + 293 cell lysate

Ab33768, at 1/250 dilution, staining eIF4E in paraffin embedded human breast carcinoma tissue sections by Immunohistochemistry.

Overlay histogram showing HEK293 cells stained with ab33768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33768, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.