Anti-EMAP II antibody [EPR14168(B)]

• Supplier: Abcam
• Catalog: ab188320
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14168(B)
• Applications: IHC-P FC ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EMAP II aa 150-250
• Description: Rabbit Monoclonal
• Gene: AIMP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-EMAP II antibody [EPR14168(B)] (ab188320) at 1/5000 dilutionLane 1 : HT-1080 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-EMAP II antibody [EPR14168(B)] (ab188320) at 1/1000 dilution + Human fetal spleen lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-EMAP II antibody [EPR14168(B)] (ab188320) at 1/1000 dilutionLane 1 : Mouse kidney lysateLane 2 : Mouse spleen lysateLane 3 : C6 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling EMAP II with ab188320 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling EMAP II with ab188320 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HT-1080 cells labeling EMAP II with ab188320 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling EMAP II with ab188320 at 1/60 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.