Anti-Emi1 antibody [EPR15320-103]

• Supplier: Abcam
• Catalog: ab187144
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15320-103
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human Emi1 aa 100-250
• Description: Rabbit Monoclonal
• Gene: FBXO5
• Conjugate: Unconjugated

Product images

All lanes : Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilutionLane 1 : Hela cell lysateLane 2 : 293 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunoprecipitation of Emi1 from HepG2 cell lysate using ab187144 at 1/50 (lane 1) or 1X PBS (negative control) (lane 2). Immunostained with ab187144 at 1/1000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Immunofluorescent analysis of HepG2 cells labeling Emi1 with ab187144 at 1/250 and DAPI staining (blue).

Immunofluorescent analysis of HeLa cells labeling Emi1 with ab187144 at 1/100 and DAPI staining (blue).

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Emi1 with ab187144 at 1/100 with a  prediluted HRP Polymer for Rabbit IgG as secondary antibody.

Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling Emi1 with ab187144 at 1/100 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.