Anti-EMSY antibody [Y474]

• Supplier: Abcam
• Catalog: ab32329
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y474
• Applications: WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
• Blocking Peptide: EMSY peptide
• Description: Rabbit Monoclonal
• Gene: C11orf30
• Conjugate: Unconjugated

Product images

Anti-EMSY antibody [Y474] (ab32329) at 1/1000 dilution + MCF7 cell lysate

Immunofluorescent analysis of EMSY expression in MCF7 cells using 1/250 ab32329.

Overlay histogram showing MCF7 cells stained with ab32329 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32329, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.