Anti-Epac1 antibody
| Name | Anti-Epac1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21236 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IP IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Synthetic peptide: DHSVLTLQLPVTASVR , corresponding to amino acids 526-541 of Epac1 |
| Description | Rabbit Polyclonal |
| Gene | RAPGEF3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab21236 staining Epac1 in mouse pancreatic acini tissue section by Immunohistochemistry (Frozen tissue sections). Tissue underwent fixation in 4% paraformaldehyde in PBS pH 7.4 for 30 minutes, PBS washed, cryoprotected and frozen with isopenatane cooled with liquid nitrogen. The primary antibody was diluted 1/200 and incubated with sample for 2 hours at room temperature. A cy3-conjugated donkey polyclonal to rabbit IgG at 1/200 dilution was used as secondary for 1 hour. Prolong Gold with 4,6-diamino-2-phenylindole was added to mounting medium to counterstain nuclei.
ICC/IF image of ab21236 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21236, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family - Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family
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TLR signaling paralyzes monocyte chemotaxis through synergized effects of p38 - TLR signaling paralyzes monocyte chemotaxis through synergized effects of p38
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Interplay among cGMP, cAMP, and Ca2+ in living olfactory sensory neurons in vitro - Interplay among cGMP, cAMP, and Ca2+ in living olfactory sensory neurons in vitro
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The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent - The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent
Thomas M, Lischka P, Muller R, Stamminger T. PLoS One. 2011;6(7):e22671.
Differential inhibitory effects of CysLT(1) receptor antagonists on P2Y(6) - Differential inhibitory effects of CysLT(1) receptor antagonists on P2Y(6)
Lau WK, Chow AW, Au SC, Ko WH. PLoS One. 2011;6(7):e22363.
Rap1 activation plays a regulatory role in pancreatic amylase secretion. - Rap1 activation plays a regulatory role in pancreatic amylase secretion.
Sabbatini ME, Chen X, Ernst SA, Williams JA. J Biol Chem. 2008 Aug 29;283(35):23884-94.
Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 - Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1
Brock TG, Serezani CH, Carstens JK, Peters-Golden M, Aronoff DM. Exp Cell Res. 2008 Jan 15;314(2):255-63. Epub 2007 Oct 23.