RAPGEF3

Gene Symbol RAPGEF3
Entrez Gene 10411
Alt Symbol CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910
Species Human
Gene Type protein-coding
Description Rap guanine nucleotide exchange factor (GEF) 3
Other Description 9330170P05Rik|EPAC 1|Rap1 guanine-nucleotide-exchange factor directly activated by cAMP|cAMP-regulated guanine nucleotide exchange factor I|exchange factor directly activated by cAMP 1|exchange protein directly activated by cAMP 1|rap guanine nucleotide exchange factor 3
Swissprots Q8WVN0 E7EQC8 A8K2G5 O95398 O95634
Accessions CAL48169 EAW57944 EAW57945 EAW57946 EAW57947 EAW57948 EAW57949 O95398 AF103905 AAC83381 AK055502 BAG51527 AK074444 AK092448 AK125545 BAG54212 AK290230 BAF82919 AK299029 BAH12932 AK308079 BC017728 AAH17728 BC040534 BC068477 BC092404 AAH92404 BQ007070 CN294139 DA340363 DA529158 DA629958 DB179165 DB278856 DQ891561 ABM82487 DQ894748 ABM85674 U78168 AAD12740 U78169 AAD02890 U79275 AAB50216 XM_005268571 XP_005268628 XM_011537751 XP_011536053 XM_011537752 XP_011536054 XM_011537753 XP_011536055 XM_011537754 XP_011536056 XM_011537755 XP_011536057 XM_011537756 XP_011536058 XM_011537757 XP_011536059 XM_011537758 XP_011536060 XM_011537759 XP_011536061 XR_944480 NM_001098531 NP_001092001 NM_001098532 NP_001092002 NM_006105 NP_006096
Function Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho- mediated signaling. Required for the actin rearrangement at cell- cell junctions, such as stress fibers and junctional actin. {ECO:0000269|PubMed:10777494, ECO:0000269|PubMed:21840392, ECO:0000269|PubMed:9853756}.
Subcellular Location Endomembrane system {ECO:0000269|PubMed:10777494}.
Tissue Specificity Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. {ECO:0000269|PubMed:9856955}.
Top Pathways Leukocyte transendothelial migration, Long-term potentiation, Adrenergic signaling in cardiomyocytes, cAMP signaling pathway, Rap1 signaling pathway

EPAC1 (5D3) Mouse mAb - 4155 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 5D3
Western blot analysis of extracts from C2C12 cells and whole rat brain using Epac1 (5D3) Mouse mAb.

Epac (A-5) - sc-28366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Epac (A-5): sc-28366 Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Epac (C-17) - sc-8880 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Epac (C-17): sc-8880. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic and nuclear staining of myocytes.

Epac (H-70) - sc-25632 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Epac (H-70): sc-25632. Western blot analysis of human recombinant Epac fusion protein.

Epac (N-16) - sc-8879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Epac (N-16): sc-8879. Western blot analysis of Epac expression in non-transfected: sc-117752 (A) and human Epac transfected: sc-113416 (B) 293T whole cell lysates.

Anti-Epac1 antibody - ab21236 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab21236 staining Epac1 in mouse pancreatic acini tissue section by Immunohistochemistry (Frozen tissue sections). Tissue underwent fixation in 4% paraformaldehyde in PBS pH 7.4 for 30 minutes, PBS washed, cryoprotected and frozen with isopenatane cooled with liquid nitrogen. The primary antibody was diluted 1/200 and incubated with sample for 2 hours at room temperature. A cy3-conjugated donkey polyclonal to rabbit IgG at 1/200 dilution was used as secondary for 1 hour. Prolong Gold with 4,6-diamino-2-phenylindole was added to mounting medium to counterstain nuclei. 

Anti-Epac1 antibody - ab124162 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of EPAC1 in rat brain tissue with ab124162 at 20 ug/mL.

Anti-Epac1 antibody - ab21235 from Abcam

Prices $373.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Epac1 antibody - ab104412 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Epac1 antibody (ab104412) at 1 µg/ml + Mouse kidney tissue lysate at 50 µg

Anti-Epac1 antibody - ab88336 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Epac1 antibody (ab88336) at 1 µg/ml + immunogen at 0.2 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution

Anti-Epac1 antibody [EPR1672] - ab109415 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1672
All lanes : Anti-Epac1 antibody [EPR1672] (ab109415) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : Human heart lysateLane 4 : HUVEC cell lysateLysates/proteins at 10 µg per lane.

Anti-Epac1 antibody [EPR1672] (Alexa Fluor® 488) - ab201506 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1672
ab201506 staining Epac1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201506 at a 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-EPAC1 delta DEP antibody - ab180737 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180737. Blue DAPI for nuclear staining.

Anti-EPAC1 delta DEP antibody - ab137901 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-EPAC1 delta DEP antibody (ab137901) at 1/500 dilution + Hela cell lysate at 30 µg

Anti-RAPGEF3 / EPAC Antibody - LS-C331978 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of U2OS cell using RAPGEF3 antibody. Blue: DAPI for nuclear staining.

Anti-RAPGEF3 / EPAC Antibody (aa740-789) - LS-C200566 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HeLa cells using RAPGEF3 antibody.

Anti-RAPGEF3 / EPAC Antibody (aa799-844) - LS-C313753 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAPGEF3 / EPAC Antibody (aa107-156) - LS-C116448 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAPGEF3 / EPAC antibody LS-C116448 Western blot of COLO205 cell lysate.

Anti-RAPGEF3 / EPAC Antibody (aa359-408) - LS-C116449 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAPGEF3 / EPAC antibody LS-C116449 Western blot of HCT15 cell lysate.

Anti-RAPGEF3 / EPAC Antibody - LS-C193104 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot.

Anti-RAPGEF3 / EPAC Antibody (C-Terminus, clone EPR1672) - LS-C138225 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1672
IHC of paraffin-embedded human kidney tissue using Anti-Epac1 antibody.

Anti-RAPGEF3 / EPAC Antibody (N-Terminus) IHC-plus™ - LS-B9589 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RAPGEF3 / EPAC antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9589 concentration 5 ug/ml.

RAPGEF3 Antibody (EPR1672) - NBP1-95559 from Novus Biologicals

Host Rabbit
Clonality Monoclonal
Clone EPR1672
Western Blot: RAPGEF3 Antibody (EPR1672) [NBP1-95559] (A) HeLa, (B) HepG2, © Human heart, and (D) HUVEC cell lysates.

RAPGEF3 Antibody - NBP1-57045 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: RAPGEF3 Antibody [NBP1-57045] - HCT15 cell lysate, concentration 0.2-1 ug/ml.

RAPGEF3 Antibody - NBP1-57044 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RAPGEF3 Antibody [NBP1-57044] - COLO205 cells lysate, concentration 0.2-1 ug/ml.
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