Anti-Eph receptor A2 antibody [3D7] (ab118882) at 1/500 dilution + Mouse brain extracts at 50 µgSecondaryGoat anti-Mouse conjugated to HRPdeveloped using the ECL technique
Human testis (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human thyroid tissue (formalin fixed, paraffin embedded) stained with ab118882 at 5 µg/ml, followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Paraffin embedded sections of Human colon cancer tissue were incubated with ab118882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Paraffin embedded sections of Human gastric cancer tissue were incubated with ab18882 (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).