All lanes : Anti-Ephrin A1 antibody (ab65072) at 1/500 dilutionLane 1 : extracts from 293 cellsLane 2 : extracts from Jurkat cellsLane 3 : extracts from Jurkat cells with immunizing peptide at 5 µgLysates/proteins at 5 µg per lane.
ab65072 at 1/500 dilution staining HeLa cells without (left) and with (right) immunizing peptide.
All lanes : Anti-Ephrin A1 antibody (ab65072) at 1 µg/mlLane 1 : Human Ephrin A1 full length protein (ab112282) at 0.1 µgLane 2 : Human Ephrin A1 full length protein (ab112282) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 150 seconds