Anti-Ephrin A3 antibody
| Name | Anti-Ephrin A3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64814 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA FC ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | A synthetic peptide derived from the C-terminal of human Ephrin A3 |
| Description | Rabbit Polyclonal |
| Gene | EFNA3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab64814 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from Jurkat cellsLane 3 : Extracts from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.