Anti-EPO Receptor antibody
| Name | Anti-EPO Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83696 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat, Goat, Horse, Guinea Pig, Bovine, Dog, Pig |
| Antigen | Synthetic peptide within residues: DHLGASLWPQ VGSLCLLLAG AAWAPPPNLP DPKFESKAAL LAARGPEELL , corresponding to N terminal amino acids 2-51 of Human EPO Receptor (NP_000112) |
| Blocking Peptide | EPO Receptor peptide |
| Description | Rabbit Polyclonal |
| Gene | EPOR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab83696 stained A498 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83696 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-EPO Receptor antibody (ab83696) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution