EPOR

Gene Symbol EPOR
Entrez Gene 281144
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description erythropoietin receptor
Other Description -
Accessions DAA27972 U61399 AAB03871 XM_005208715 XP_005208772 NM_001205601 NP_001192530
Top Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Hematopoietic cell lineage, PI3K-Akt signaling pathway

Anti-EPO Receptor antibody - ab83696 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab83696 stained A498 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83696 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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