Anti-ERAB antibody

• Supplier: Abcam
• Catalog: ab52243
• Prices: $385.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB ELISA IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from human ERAB
• Description: Rabbit Polyclonal
• Gene: HSD17B10
• Conjugate: Unconjugated

Product images

All lanes : Anti-ERAB antibody (ab52243) at 1/300 dilutionLane 1 : LOVO cell extract; without peptideLane 2 : LOVO cell extract; with peptide

Ab52243 staining human normal lung tissue. Staining is localised to mitochondria.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ICC/IF image of ab55243 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Ovarian hormone loss induces bioenergetic deficits and mitochondrial - Ovarian hormone loss induces bioenergetic deficits and mitochondrial

Yao J, Irwin R, Chen S, Hamilton R, Cadenas E, Brinton RD. Neurobiol Aging. 2012 Aug;33(8):1507-21. doi:

Mitochondrial bioenergetic deficit precedes Alzheimer's pathology in female mouse - Mitochondrial bioenergetic deficit precedes Alzheimer's pathology in female mouse

Yao J, Irwin RW, Zhao L, Nilsen J, Hamilton RT, Brinton RD. Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14670-5. doi: