All lanes : Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilutionLane 1 : A431 cell lysate, untreatedLane 2 : A431 cell lysate, treated with Epidermal Growth Factor (EGF) Lysates/proteins at 10 µg/ml per lane.
ab109273 at 1/10 dilution staining ErbB 4 in HeLa cells by Flow cytometry. Green: Permeabilized A431 cells, untreated Red: Permeabilized A431 cells, treated with EGF. Blue: Permeabilized A431 cells, treated with EGF and preincubated with phospho-ErbB 4 (pY1284) peptide Orange: Permeabilized A431 cells, treated with EGF and preincubated with non-phospho-ErbB 4(pY1284) peptide.
Immunofluorescent staining of A431 cells using ab109273 at a dilution of 1/100.