All lanes : Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293T cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells labeling ERO1L with ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).