Western blot analysis on immunoprecipitation pellet from (1) HepG2 cell lysate or (2) 1X PBS (negative control) using ab172954 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Note: primary antibody diluted with 1% BSA.
Flow cytometric analysis of permeabilized 293T cells using ab172954 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
All lanes : Anti-ERO1L antibody [EPR12475(B)] (ab172954) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLane 5 : C6 cell lysateLane 6 : RAW 264.7 cell lysateLane 7 : PC-12 cell lysateLysates/proteins at 10 µg per lane.