Anti-EST1A antibody
| Name | Anti-EST1A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab87539 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human EST1A |
| Description | Rabbit Polyclonal |
| Gene | SMG6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-EST1A antibody (ab87539) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab87539 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab87539, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100% methanol fixed (5 min) MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data
Product References
Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA - Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA
Li T, Shi Y, Wang P, Guachalla LM, Sun B, Joerss T, Chen YS, Groth M, Krueger A, Platzer M, Yang YG, Rudolph KL, Wang ZQ. EMBO J. 2015 Mar 14. pii: e201489947.
An atlas of chromatoid body components. - An atlas of chromatoid body components.
Meikar O, Vagin VV, Chalmel F, Sostar K, Lardenois A, Hammell M, Jin Y, Da Ros M, Wasik KA, Toppari J, Hannon GJ, Kotaja N. RNA. 2014 Apr;20(4):483-95.
The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation - The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation
Fatscher T, Boehm V, Weiche B, Gehring NH. RNA. 2014 Oct;20(10):1579-92.