Anti-Estrogen Receptor beta antibody
| Name | Anti-Estrogen Receptor beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3576 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF WB B/N ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Sheep, Human, Pig, Primate |
| Antigen | Synthetic peptide corresponding to Rat Estrogen Receptor beta aa 467-485 |
| Description | Rabbit Polyclonal |
| Gene | ESR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
All lanes : Anti-Estrogen Receptor beta antibody (ab3576) at 1/2000 dilutionLane 1 : CAL region of Human brain - Whole cell lysateLane 2 : EC region of Human brain - Whole cell lysateLane 3 : HIP region of Human brain - Whole cell lysateLane 4 : IT region of Human brain - Whole cell lysateLane 5 : MF region of Human brain - Whole cell lysateLane 6 : SP region of Human brain - Whole cell lysateSecondaryHRP conjugated Donkey Anti-rabbit IgG
Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).
Immunofluorescence analysis of frozen murine thoracic aorta, staining Estrogen Receptor beta with ab3576 at 1/50 dilution (green: not merged with nucleus, or purple: merged with nucleus). Nuclei were stained with DAPI (blue) and actin fibers were stained red.
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