ESR2

Gene Symbol ESR2
Entrez Gene 2100
Alt Symbol ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2
Species Human
Gene Type protein-coding
Description estrogen receptor 2 (ER beta)
Other Description estrogen receptor beta|estrogen receptor beta 4|nuclear receptor subfamily 3 group A member 2
Swissprots O60702 Q0MWT5 Q86Z31 A8K8K5 O75584 O75583 O60685 Q92731 Q9UEV6 O60703 O60608 Q0MWT6 Q9UHD3 G3V5M5 Q9UQK9
Accessions AAG29940 AAV31779 EAW80845 EAW80846 EAW80847 EAW80848 EAW80849 EAW80850 EAW80851 EAW80852 EAW80853 EAW80854 Q92731 AB006589 BAA31966 AB006590 BAA24953 AB209620 BAD92857 AF047463 AF051427 AAC05985 AF051428 AAC05751 AF060555 AAC15234 AF061054 AAC39784 AF061055 AAC39785 AF074598 AAC25602 AF074599 AAC25603 AF124790 AAD32580 AK292370 BAF85059 AY438022 BC024181 AAH24181 BX094834 BX247957 CAD62297 CN366005 DQ777076 ABG88022 DQ777077 DQ838582 ABH09189 DQ838583 ABH09190 HG506303 HG506304 HQ692820 ADZ17331 HQ692821 ADZ17332 KC777381 KC777382 KC777383 KC777384 KC777385 KC777386 KC777387 KC777388 KJ534833 AHW56473 X99101 CAA67555 XM_011536545 XP_011534847 XM_011536546 XP_011534848 NM_001040275 NP_001035365 NM_001214902 NP_001201831 NM_001271876 NP_001258805 NM_001271877 NP_001258806 NM_001291712 NP_001278641 NM_001291723 NP_001278652 NM_001437 NP_001428 NR_073496 NR_073497 NM_001040276 NM_001214903
Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:19126643}.
Tissue Specificity Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
Top Pathways Prolactin signaling pathway, Estrogen signaling pathway

Estrogen Receptor β Antibody - 5513 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines and tissues using Estrogen Receptor β Antibody.

ERβ (1531) - sc-53494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of ERβ phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-ERβ (Ser 87): sc-32826 (A,B) and ERβ (1531): sc-53494 (C,D).

ERβ (L-20) - sc-6822 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ERβ (N-19) - sc-6820 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ERβ (N-19): sc-6820. Western blot analysis of ERβ expression in mouse liver tissue extract.

ERβ (H-150) - sc-8974 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ERβ (H-150): sc-8974. Western blot analysis of ERβ expression in I-11.15 (A), F9 (B) and NIH/3T3 (C) whole cell lysates and mouse thymus (D) and mouse spleen (E) tissue extracts.

p-ERβ (Ser 87) - sc-32826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of ERβ phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-ERβ (Ser 87): sc-32826 (A,B) and ERβ (1531): sc-53494 (C,D).

Anti-Estrogen Receptor beta (phospho S105) antibody - ab62257 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62257 at 1/500 - 1/1000 dilution staining Estrogen Receptor beta in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide

Anti-Estrogen Receptor beta antibody - ab3576 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis using Estrogen Receptor beta polyclonal antibody (ab3576) shows nuclear localization of Estrogen Receptor beta in MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Estrogen Receptor beta (ab3576), at a dilution of 1:100 for 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Anti-Estrogen Receptor beta antibody - ab5786 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining with ab5786 detects nuclear Estrogen Receptor beta in rat prostate

Anti-Estrogen Receptor beta antibody - ab5784 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Estrogen Receptor beta (green) with ab5784 at 1/100. Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were incubated with the primary antibody for at least 1 hour at room temperature. A DyLight 488-conjugated goat anti-rabbit IgG (1/200) secondary antibody was used. Nuclei (blue) were stained with Hoechst. 20X magnification.

Anti-Estrogen Receptor beta antibody - ab196787 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Estrogen Receptor beta antibody (ab196787) at 1/500 dilutionLane 1 : BT474 cell extractsLane 2 : Raji cell extracts

Anti-Estrogen Receptor beta antibody - ab78946 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Estrogen Receptor beta antibody (ab78946) at 1/500 dilutionLane 1 : Extracts from HeLa cells treated with EGF (200ng/ml, 30mins)Lane 2 : Extracts from HeLa cells treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Estrogen Receptor beta antibody - ab1103 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-Estrogen Receptor beta antibody - ab140438 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Estrogen Receptor beta antibody (ab140438) at 1 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Estrogen Receptor beta antibody - ab111086 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab111086, at 3.75µg/ml, staining Estrogen Receptor beta in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Anti-Estrogen Receptor beta antibody [14C8] - ab288 from Abcam

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 14C8
ab288 staining endocervical epithelium by IHC-P.

Anti-Estrogen Receptor beta antibody [4D2] - ab485 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D2

Anti-Estrogen Receptor beta antibody [6A12] - ab286 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6A12

Anti-Estrogen Receptor beta antibody [7B10.7] - ab782 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 7B10.7

Anti-Estrogen Receptor beta antibody [EPR3777] - ab92306 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3777
All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) at 1/1000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit antibody at 1/2000 dilution

Anti-Estrogen Receptor beta antibody [EPR3777] (HRP) - ab200599 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3777
All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (HRP) (ab200599) at 1/5000 dilutionLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Estrogen Receptor beta antibody [EPR3778] - ab133467 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3778
ab133467 staining Estrogen Receptor beta in Human ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/900). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

Anti-Estrogen Receptor beta antibody [PPG5/10] - ab27720 from Abcam

Prices $378.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone PPG5/10
Paraffin embedded human ovary tissue section immunostained for Estrogen receptor beta using monoclonal antibody ab27720

Anti-ESR2 / ER Beta Antibody (aa369-397) - LS-C159571 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
ESR2 Antibody western blot of K562 cell line lysates (35 ug/lane). The ESR2 antibody detected the ESR2 protein (arrow).

Anti-ESR2 / ER Beta Antibody (aa369-397) - LS-C159572 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
ESR2 Antibody (Ascites)western blot of K562 cell line lysates (35 ug/lane). The ESR2 antibody detected the ESR2 protein (arrow).
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