Anti-Estrogen Receptor beta antibody [EPR3778]

• Supplier: Abcam
• Catalog: ab133467
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3778
• Applications: WB IHC-P
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues on the C terminus in Human Estrogen Receptor beta (UniProt Q92731)
• Description: Rabbit Monoclonal
• Gene: ESR2
• Conjugate: Unconjugated

Product images

ab133467 staining Estrogen Receptor beta in Human ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/900). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

All lanes : Anti-Estrogen Receptor beta antibody [EPR3778] (ab133467) at 1/1000 dilutionLane 1 : MCF-7 Cell LysateLane 2 : 293 Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

All lanes : Anti-Estrogen Receptor beta antibody [EPR3778] (ab133467) at 1/1000 dilution (unpurified)Lane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of Estrogen Receptor beta in paraffin embedded Human testis tissue, using ab133467, unpurified, at a dilution of 1/100.