Anti-ETFA antibody [2B11AE8]

Name	Anti-ETFA antibody [2B11AE8]
Supplier	Abcam
Catalog	ab110316
Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	2B11AE8
Applications	IP WB ICC/IF ICC/IF ELISA FC IHC-P
Species Reactivities	Mouse, Rat, Human
Antigen	Human recombinant full length protein ETFA
Description	Mouse Monoclonal
Gene	ETFA
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix

All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/mlLane 1 : Human heart tissue Lane 2 : HepG2 whole cells Lane 3 : Human liver mitochondriaLane 4 : Bovine heart mitochondria Lane 5 : Rat liver mitochondriaLane 6 : Mouse liver mitochondria Lysates/proteins at 10 µg per lane.

HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Complex I function and supercomplex formation are preserved in liver mitochondria - Complex I function and supercomplex formation are preserved in liver mitochondria

Davoudi M, Kotarsky H, Hansson E, Fellman V. PLoS One. 2014 Jan 22;9(1):e86767.

Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid - Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid

Pougovkina O, te Brinke H, Ofman R, van Cruchten AG, Kulik W, Wanders RJ, Houten SM, de Boer VC. Hum Mol Genet. 2014 Jul 1;23(13):3513-22.