ETFA

Gene Symbol ETFA
Entrez Gene 2108
Alt Symbol EMA, GA2, MADD
Species Human
Gene Type protein-coding
Description electron-transfer-flavoprotein, alpha polypeptide
Other Description alpha-ETF|electron transfer flavoprotein alpha-subunit|electron transfer flavoprotein subunit alpha, mitochondrial|electron transfer flavoprotein, alpha polypeptide|glutaric aciduria II
Swissprots Q53XN3 B4DT43 P13804
Accessions AAN03712 CAA11802 EAW99221 EAW99222 P13804 AA557337 AK292979 BAF85668 AK300044 BAG61855 BC015526 AAH15526 BC029995 BC034156 BC095457 AAH95457 BG261160 BT009796 AAP88798 BU176141 D28380 BAA05746 DA838974 J04058 AAA52406 XR_931766 NM_000126 NP_000117 NM_001127716 NP_001121188
Function The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Subcellular Location Mitochondrion matrix.

ETFA (C-17) - sc-66219 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ETFA (C-17): sc-66219. Western blot analysis of ETFA expression in K-562 whole cell lysate.

ETFA (E-18) - sc-66220 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ETFA (H-8) - sc-365982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ETFA (H-8): sc-365982. Western blot analysis of ETFA expression in A549 whole cell lysate.

ETFA (H-157) - sc-366893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-ETFA antibody - ab153722 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ETFA antibody (ab153722) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-ETFA antibody - ab126131 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab126131, at 1/100 dilution (green), staining methanol fixed HeLa cells by immunofluorescence.

Anti-ETFA antibody - ab85150 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab85150, at a 1/100 dilution, staining ETFA in human fetal stomach tissue.

Anti-ETFA antibody [2B11AE8] - ab110316 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B11AE8
Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix

Anti-ETFA antibody [2B11AE8] (Alexa Fluor® 488) - ab198303 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B11AE8
ab198303 staining ETFA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198303 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Anti-ETFA antibody [2B11AE8] (Alexa Fluor® 647) - ab198104 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B11AE8
ab198104 staining ETFA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198104 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Anti-ETFA antibody [2B11AE8] (HRP) - ab198304 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B11AE8
IHC image of ETFA staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198304 at 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-ETFA antibody - C-terminal - ab189970 from Abcam

Prices $407.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ETFA antibody - C-terminal (ab189970) at 1/1000 dilutionLane 1 : K562 cell line lysatesLane 2 : Mouse liver tissue lysatesLysates/proteins at 35 µg per lane.

Anti-ETFA Antibody (aa283-311, AP) - LS-C222472 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (aa283-311, APC) - LS-C222473 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (aa283-311, Biotin) - LS-C222474 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (Internal) - LS-C137139 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (N-Terminus) - LS-C81833 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ETFA Antibody (aa139-152) - LS-C139565 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ETFA antibody (0.1 ug/ml) staining of Human Colon lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ETFA Antibody (aa218-267) - LS-C81834 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ETFA Antibody (aa283-311, Azide-free, HRP) - LS-C222476 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (aa283-311, PE) - LS-C222477 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (clone 2A5) - LS-C197161 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2A5
Detection limit for recombinant GST tagged ETFA is 1 ng/ml as a capture antibody.

Anti-ETFA Antibody (aa283-311, FITC) - LS-C222475 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (aa34-295, Biotin) - LS-C297343 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ETFA Antibody (aa34-295, Biotin) - LS-C297344 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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