Anti-EVI1 antibody
| Name | Anti-EVI1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28457 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog |
| Antigen | Synthetic peptide derived from residues 964 - 1013 of human EVI1 |
| Description | Rabbit Polyclonal |
| Gene | MECOM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-EVI1 antibody (ab28457) at 0.5 µg/ml + fetal lung lysate at 10 µgSecondary HRP conjugated anti rabbit IgG at 1/50000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling EVI1 with ab28457 at 4-8µg/ml. Cells with positive label: epithelial cells of intestinal villus (indicated with arrows). Magnification: 400X.
ICC/IF image of ab28457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.