Anti-EWSR1 antibody
| Name | Anti-EWSR1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54708 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | IHC-P ICC/IF ICC/IF IP WB |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: SDNSAIYVQG LNDSVTLDDL ADFFKQCGVV KMNKRTGQPM IHIYLDKETG KPKGDATVSY EDPPTAKAAV EWFDGKDFQG SKLKVSLARK KPPMNS, corresponding to amino acids 358-454 of Human EWSR1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | EWSR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab54708 stained hepG2 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
EWSR1 antibody (ab54708) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human urinary bladder.
Western blot against tagged recombinant protein immunogen using ab54708 EWSR1 antibody at 1ug/ml.