EWSR1

Gene Symbol EWSR1
Entrez Gene 2130
Alt Symbol EWS, bK984G1.4
Species Human
Gene Type protein-coding
Description EWS RNA-binding protein 1
Other Description EWS RNA-binding protein variant 6|Ewing sarcoma breakpoint region 1|Ewings sarcoma EWS-Fli1 (type 1) oncogene|RNA-binding protein EWS
Swissprots Q96FE8 Q96MN4 Q01844 Q96MX4 Q92635 B0QYK1 Q9BWA2 Q5THL0
Accessions AAD15010 BAA31990 CAA51489 CAA69177 CAA70044 CAH33891 EAW59780 EAW59781 EAW59782 EAW59783 EAW59784 EAW59785 EAW59786 EAW59787 Q01844 AI564543 AK026270 AK056309 BAB71145 AK056681 BAB71252 AK096787 AK127624 AK225096 AK225102 AL833489 AW205707 BC000527 AAH00527 BC004817 AAH04817 BC011048 AAH11048 BC072442 AAH72442 BE741903 BM981230 BX648769 CR456490 CAG30376 CU013087 CAK54518 CU013375 CAK54817 CX786322 DA013931 DA051447 DB051511 DB443651 EU327511 ABY40719 JX977847 AGS36504 S62665 AAB27172 X66899 CAA47350 XM_005261389 XP_005261446 XM_005261390 XP_005261447 XM_011529995 XP_011528297 XM_011529996 XP_011528298 XM_011529997 XP_011528299 XM_011529998 XP_011528300 XM_011529999 XP_011528301 XM_011530000 XP_011528302 XM_011530001 XP_011528303 XM_011530002 XP_011528304 NM_001163285 NP_001156757 NM_001163286 NP_001156758 NM_001163287 NP_001156759 NM_005243 NP_005234 NM_013986 NP_053733
Function Might normally function as a transcriptionnal repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Subcellular Location Nucleus {ECO:0000269|PubMed:16965792}. Cytoplasm {ECO:0000269|PubMed:16965792}. Cell membrane {ECO:0000269|PubMed:16965792}. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
Tissue Specificity Ubiquitous.
Top Pathways Transcriptional misregulation in cancer

EWS Antibody - 11910 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using EWS Antibody.

EWS (B-1) - sc-398318 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EWS (B-1): sc-398318. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear, cytoplasmic, membrane and nucleolar localization.

EWS (N-18) - sc-6533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EWS (N-18): sc-6533. Western blot analysis of EWS expression in K-562 (A) and C32 (B) whole cell lysates.

EWS (C-19) - sc-6532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EWS (C-19): sc-6532. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.

EWS (G-5) - sc-28327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EWS (G-5): sc-28327. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

EWS (H-60) - sc-28865 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EWS (H-60): sc-28865. Western blot analysis of EWS expression in non-transfected 293: sc-110760 (A), human EWS transfected 293: sc-111059 (B) and Jurkat (C) whole cell lysates.

EWS (C-9) - sc-48404 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EWS (C-9): sc-48404. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Anti-EWSR1 antibody - ab93837 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab93837 (1/200) staining EWSR1 in assynchronous HeLa Cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview

Anti-EWSR1 antibody - ab54708 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of ab54708 stained hepG2 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-EWSR1 antibody - ab81971 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemical staining of paraffin embedded human small intestine with ab81971 at 3.8 µg/mL. Heat mediated antigen retrieval was carried out with citrate pH 6.

Anti-EWSR1 antibody - ab93464 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of U251 cells labelling EWSR1 with ab93464. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab93464 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EWSR1 immunoreactivity is localized to the nucleus.

Anti-EWSR1 antibody - ab153719 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-EWSR1 antibody (ab153719) at 1/1000 dilution + A549 whole cell lysate at 30 µg

Anti-EWSR1 antibody - ab84389 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84389, at 1/250 dilution, staining EWSR1 in formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue by Immunohistochemistry, using DAB staining and an anti-Rabbit HRP conjugated secondary antibody at a dilution of 1/100.

Anti-EWSR1 antibody - ab36796 from Abcam

Prices $370.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-EWSR1 antibody - ab202383 from Abcam

Prices $359.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-EWSR1 antibody (ab202383) at 1/1000 dilution + NIH3T3 cell line lysate at 20 µgSecondaryGoat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

Anti-EWSR1 antibody [5C10] - ab124404 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5C10
All lanes : Anti-EWSR1 antibody [5C10] (ab124404) at 1/500 dilutionLane 1 : EWSR1-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysate

Anti-EWSR1 antibody [5H7] - ab125088 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H7
Anti-EWSR1 antibody [5H7] (ab125088) at 1/1000 dilution + HeLa cell line lysate

Anti-EWSR1 antibody [EPR4647] - ab133288 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4647
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling EWSR1 with ab133288 at 1/250 dilution.

Anti-EWSR1 antibody [EPR4648] - ab133318 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4648
All lanes : Anti-EWSR1 antibody [EPR4648] (ab133318) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : F9 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-EWSR1 / EWS Antibody (aa100-150) - LS-C65791 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EWSR1 / EWS Antibody (aa173-222) - LS-C80367 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.

Anti-EWSR1 / EWS Antibody (C-Terminus) - LS-C110293 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EWSR1 antibody LS-C110293 Western blot of Jurkat lysate.

Anti-EWSR1 / EWS Antibody (aa216-265) - LS-C110562 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EWSR1 antibody LS-C110562 Western blot of HepG2 cell lysate.

Anti-EWSR1 / EWS Antibody (aa623-652, AP) - LS-C256863 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-EWSR1 / EWS Antibody (aa623-652, APC) - LS-C256864 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more