Immunofluorescence analysis of U251 cells labelling EWSR1 with ab93464. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab93464 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EWSR1 immunoreactivity is localized to the nucleus.
Flow cytometry analysis of HeLa cells (right histogram) labelling EWSR1 with ab93464, compared to a negative control (left histogram). A FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
Anti-EWSR1 antibody (ab93464) at 1/100 dilution + A2058 cell line lysates at 35 µg