All lanes : Anti-EXOSC1 antibody [EPR13526] (ab181108) at 1/2000 dilutionLane 1 : K562 cell lysateLane 2 : C6 cell lysateLane 3 : Raw264.7 cell lysateLane 4 : PC-12 cell lysateLane 5 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling EXOSC1 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.
Western blot analysis of EXOSC1 in immunoprecipitation pellets from Raji lysate. ab181108 used at 1/60 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Lane 2 shows negative control.
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling EXOSC1 with ab181108 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488), Conterstained with DAPI blue.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EXOSC1 with ab181108 at 1/100 dilution. Counter stain Hematoxylin.
All lanes : Anti-EXOSC1 antibody [EPR13526] (ab181108) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : 293 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution