All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilutionLane 1 : Mouse kidney tissue lysateLane 2 : Mouse spleen tissue lysateLane 3 : Rat kidney tissue lysateLane 4 : Rat spleen tissue lysateLane 5 : RAW264.7 cell line lysateLane 6 : NIH 3T3 cell line lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.
Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.
Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin.
All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilutionLane 1 : Jurkat cell line lysateLane 2 : HeLa cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution