All lanes : Anti-Exosome Component 9 antibody (ab156686) at 0.1 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
All lanes : Anti-Exosome Component 9 antibody (ab156686) at 0.4 µg/mlLane 1 : Mouse NIH3T3 whole cell lysate at 50 µgLane 2 : Mouse NIH3T3 whole cell lysate at 15 µgdeveloped using the ECL technique
Detection of Exosome Component 9 by Western Blot of Immunoprecipitate. Immunoprecipitation of 293T whole cell lysate using ab156686 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection 9 with ab156686 at 0.4 µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.