Anti-Exportin-5 antibody [EPR8452]

• Supplier: Abcam
• Catalog: ab129006
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8452
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human Exportin-5
• Description: Rabbit Monoclonal
• Gene: XPO5
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab129006 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129006, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Exportin-5 antibody [EPR8452] (ab129006) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Human fetal heart lysateLane 4 : Human fetal brain lysateLane 5 : C6 cell lysateLane 6 : RAW264.7 cell lysateLane 7 : PC12 cell lysateLane 8 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

ab129006, at 1/250 dilution, staining Exportin-5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD