Anti-Factor I antibody

• Supplier: Abcam
• Catalog: ab8843
• Prices: $378.00
• Sizes: 1 ml
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA ICC/IF ICC/IF Immunodiffusion Immunoelectrophoresis RID
• Species Reactivities: Human
• Antigen: Human Factor I purified from plasma
• Description: Sheep Polyclonal
• Gene: CFI
• Conjugate: Unconjugated

Product images

ICC/IF image of ab8843 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8843 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- Sheep (ab150177) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Product References

Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. - Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome.

Lemaire M, Fremeaux-Bacchi V, Schaefer F, Choi M, Tang WH, Le Quintrec M, Fakhouri F, Taque S, Nobili F, Martinez F, Ji W, Overton JD, Mane SM, Nurnberg G, Altmuller J, Thiele H, Morin D, Deschenes G, Baudouin V, Llanas B, Collard L, Majid MA, Simkova E, Nurnberg P, Rioux-Leclerc N, Moeckel GW, Gubler MC, Hwa J, Loirat C, Lifton RP. Nat Genet. 2013 May;45(5):531-6.

Mutations in components of complement influence the outcome of Factor - Mutations in components of complement influence the outcome of Factor

Bienaime F, Dragon-Durey MA, Regnier CH, Nilsson SC, Kwan WH, Blouin J, Jablonski M, Renault N, Rameix-Welti MA, Loirat C, Sautes-Fridman C, Villoutreix BO, Blom AM, Fremeaux-Bacchi V. Kidney Int. 2010 Feb;77(4):339-49.