Anti-Factor VII antibody
| Name | Anti-Factor VII antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab97614 |
| Prices | $385.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Human, Monkey |
| Antigen | Recombinant protein fragment corresponding to a region within amino acids 209 and 444 of Human Factor VII |
| Description | Rabbit Polyclonal |
| Gene | F7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab97614 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97614, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-Factor VII antibody (ab97614) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Anti-Factor VII antibody (ab97614) at 1/500 dilution + H1299 whole cell lysate at 30 µg
Immunohistochemistry analysis of paraffin-embedded N human (gastric carcinoma) tissue, using ab97614 at 1/100 dilution.