Anti-FADD antibody [EP887Y]

Name	Anti-FADD antibody [EP887Y]
Supplier	Abcam
Catalog	ab52935
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EP887Y
Applications	ICC/IF ICC/IF WB IHC-P
Species Reactivities	Mouse, Rat, Human
Antigen	A synthetic peptide corresponding to residues near the N-term of human FADD
Description	Rabbit Monoclonal
Gene	FADD
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ab52935 staining FADD in the A549 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab52935 staining FADD in Rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

ab52935 staining FADD in Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-FADD antibody [EP887Y] (ab52935) at 1/1000 dilution + NIH/3T3 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Anti-FADD antibody [EP887Y] (ab52935) at 1/500 dilution (unpurified) + Jurkat cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit

Immunohistochemical analysis of paraffin-embedded human lung tissue using ab52935, unpurified, at a dilution of 1/100-1/250.Immunohistochemical analysis of paraffin-embedded human lung tissue using ab52935 at a dilution of 1/100-1/250.

Product References

FADD regulates thymocyte development at the beta-selection checkpoint by - FADD regulates thymocyte development at the beta-selection checkpoint by

Zhang X, Dong X, Wang H, Li J, Yang B, Zhang J, Hua ZC. Cell Death Dis. 2014 Jun 5;5:e1273.

Effects of ethanol on transforming growth factor Beta1-dependent and -independent - Effects of ethanol on transforming growth factor Beta1-dependent and -independent

Hicks SD, Miller MW. Exp Neurol. 2011 Jun;229(2):372-80.

Fas activation in alveolar epithelial cells induces KC (CXCL1) release by a - Fas activation in alveolar epithelial cells induces KC (CXCL1) release by a

Farnand AW, Eastman AJ, Herrero R, Hanson JF, Mongovin S, Altemeier WA, Matute-Bello G. Am J Respir Cell Mol Biol. 2011 Sep;45(3):650-8.

Signaling pathway via TNF-alpha/NF-kappaB in intestinal epithelial cells may be - Signaling pathway via TNF-alpha/NF-kappaB in intestinal epithelial cells may be

Onizawa M, Nagaishi T, Kanai T, Nagano K, Oshima S, Nemoto Y, Yoshioka A, Totsuka T, Okamoto R, Nakamura T, Sakamoto N, Tsuchiya K, Aoki K, Ohya K, Yagita H, Watanabe M. Am J Physiol Gastrointest Liver Physiol. 2009 Apr;296(4):G850-9. doi: