Anti-FAK (phospho S732) antibody
| Name | Anti-FAK (phospho S732) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4792 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human, Chicken, Xenopus |
| Antigen | Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains serine 732 |
| Description | Rabbit Polyclonal |
| Gene | PTK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence labelling of FAK (phospho S732) with antibody ab4792 on mouse Cor 1 cells. Fixative: Formaldehyde. Permeabilization: Tween 20. Blocking step: 1% BSA for 10 mins @ rt°C. ab4792 used at 1/200 for 2 hours in TBS/BSA/azide/Tween. Secondary antibody: anti Rabbit IgG Alexa Fluor® 594 (1/1000). Nuclear positivity of mitotic cells is observed as well as a weaker punctate cytoplasmic positivity. The submitted composite image shows an intensely stained nucleus (upper image) that is clearly a cell in anaphase (lower image of Hoechst staining). To the right side of the upper image, three nuclei exhibit varying degrees of positivity: perhaps these cells are about to enter/leave the mitotic cycle.See Abreview
Immunohistochemistical detection of FAK (phospho S732) using antibody (ab4792) on Formaldehyde-fixed paraffin-embedded mosue ovary sections. Antigen retrieval step: Heat mediated; Buffer used: Citric acid pH6; Blocking step: 1% BSA for 10 mins @ rt°C. ab4792 (1/200) incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG conjugated to biotin (1/200). The image shows clear nuclear positivity in the middle secondary follicle specific to mitotic cells.See Abreview
Product References
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